Project on a Biochip-Based Olfactory System
olfactory system is a collaborative project to develop a bioelectronic
device that can detect chemicals with high sensitivity. This device
makes use of olfactory neurons from dogs. The neurons will be connected
to the input of a digital processorvia a microelectrode. The signal
of the neurons will be analyzed by various methods including pattern
recognition and neural network processing. CAMP Professor Maciej
Markowski is responsible for developing a protocol for growing olfactory
neurons and stem cells on the microelectrode. CAMP Professor Anja
Mueller will use a biodegradable polymer to manage the connection
of the neurons to the contact areas of the microelectrode. Professors
Antonio Rubio and Jose-Luis Gonzalez, of the Electrical Engineering
Department at U.P.C. in Barcelona, Spain, will develop the analysis
of the neuron signals. Also CAMP Professor Richard Partch will work
on a polymer for encasing the device, allowing water to be contained
and oxygen to be exchanged.
7: A Biochip-Based Olfactory System
Egon Matijevic' Prepares Nano- and Macro- Sized Particles for Medical
Matijevic', the Victor K. LaMer Chair of Colloid and Surface Science
at Clarkson University, and his group are synthesizing and characterizing
uniform particles in sizes ranging from nanometers to micrometers
for medical diagnostics. Two examples of this work are described.
1. Stable aqueous
dispersions consisting of CdS nanoparticles, having modal diameters
ranging between 2 and 8 nm, were prepared with amino-derivatized
polysaccharides (aminodextrans, Amdex) as the stabilizing agents.
These Amdex-CdS nanoparticle complexes could be activated and conjugated
with antibody by conventional means.
1. The purified
conjugate of the aminodextran-CdS nanoparticle complex with anti-CD4
monoclonal antibody was then mixed with a whole blood control, followed
by indirect sheep animouse antibody - phycoerythrin (SAM-PE) labeling
of washed cells incubated with T4-5X-Amdex-CdS. Red blood cells
were then lysed and quenched and the resulting mixture, which was
run on a flow cytometer with 488 nm argon ion laser excitation,
suggested that the T4 antibody from the conjugate was present specifically
fluorescent microspheres of silica-dye have been prepared by coating
preformed monodispersed silica particles with silica layers containing
rhodamine 6G or acridine orange. The resulting dispersions exhibit
intense fluorescence emission between 500 and 600 nm, over a broad
excitation wavelength range of 460 to 550 nm, even with exceeding
small amounts of dyes incorporated into the silica particles (10
- 30 ppm, expressed as the weight of the dye relative to the weight
of the dry particles). The fluorescent particles can be prepared
in micrometer diameters suitable for analyses using flow cytometry
with 488 nm laser excitation.
more information about Professor Matijevic' and his research,
you may call him at 315-268-2392 or send email to email@example.com.
Professor Ian Suni Works to Develop Biosensors for Detecting Biological
Ian Suni, in collaboration with Professor Linda Luck from Clarkson
University's Departments of Chemistry and Biology, is working on
the development of biosensors for the detection of biological warfare
agents, environmental toxins, and other proteins of analytical interest
to the field of biotechnology. These biosensors involve surface
arrays of chemisorbed proteins that undergo biological recognition
events which induce detectable changes in mass, voltage and fluorescence.
Natural proteins can also be altered by site-specific mutagenesis
to introduce functional groups that improve detectability.
more information about Professor Suni and his research, you
may call him at 315-268-4471 or send emailto firstname.lastname@example.org.