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Costel C. Darie

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darie

Costel C. Darie
Assistant Professor
Chemistry & Biomolecular Science
147 Science Center
Clarkson University
PO Box 5810
Potsdam, NY 13699-5810
Phone: 315-268-7763
E-mail: cdarie@clarkson.edu

Website:
http://people.clarkson.edu/~cdarie/index.html

Educational Background

2002 Ph.D., Biochemistry, University of Freiburg, Germany
1999 MS, Biochemistry, Al.I. Cuza University, Iasi, Romania
1997 BS, Biochemistry, Al.I. Cuza University, Iasi, Romania
1991 RN, Vaslui County Hospital, Vaslui, Romania


Professional Experience

2008-2009    Associate Scientist, Mount Sinai School of Medicine, New York, NY, USA
2006-2008    Research Scientist, Skirball Institute, New York University, New York, NY, USA
2003-2006    Postdoctoral Associate, Mount Sinai School of Medicine, New York, NY, USA


Research Interests
Our research interests focus on three main areas: 1) understanding the molecular mechanisms of protein-protein interactions and how they potentially relate to disease states and 2) investigating the molecular mechanisms of cell proliferation and differentiation 3) identifying serum biomarkers for diagnostic and prognostic of cancer. In our studies, we mainly use a combination of analytical biochemistry, protein chemistry and mass spectrometry.
 

Selected Publications

  
Sokolowska, I., Gawinowicz, M.A., Ngounou Wetie, A.G., Darie, C.C.(2012). Disulfide proteomics for identification of extracellular or secreted proteins. Electrophoresis. In Press. 

Sokolowska, I., Dorobantu, C., Woods, A.G., Macovei, A., Branza-Nichita N, Darie, C.C.(2012). Proteomic analysis of plasma membranes isolated from undifferentiated and differentiated HepaRG cells. Proteome Science. In Press.
 
Sokolowska, I., Woods, A.G., Gawinowicz, M.A., Roy, U., Darie, C.C. (2012). Identification of a potential tumor differentiation factor (TDF) receptor candidate in prostate cancer cells. FEBS Journal. Accepted. DOI: 10.1111/j.1742-4658.2012.08641.x
  
Woods, A.G., Sokolowska, I., Darie, C.C.(2012). Identification of consistent alkylation of cysteine-less peptides in a proteomics experiment. Biochemical and Biophysical Research Communications, 419 (2): 305-8. 

Sokolowska, I., Woods, A.G., Gawinowicz, M.A., Roy, U., Darie, C.C.(2012). Identification of a potential tumor differentiation factor (TDF) receptor from steroid-responsive and steroid-resistant breast cancer cells., J. Biol. Chem., 287(3):1719-33.
 
Woods A.G., Sokolowska I., Taurines R., Gerlach M., Dudley E., Thome J., Darie C.C. (2012). Potential biomarkers in psychiatry: focus on the cholesterol system. doi: 10.1111/j.1582-4934.2012.01543.
 
Thome J., Coogan A.N., Woods A.G., Darie C.C., Häßler F. (2011). CLOCK Genes and Circadian Rhythmicity in Alzheimer Disease. J Aging Res., 2011:383091 doi:10.4061/2011/383091.
 
Sokolowska, I., Woods, A.G., Wagner, J., Dorler, J., Wormwood, K., Thome, J., Darie, C.C. (2011). Mass spectrometry for proteomics-based investigation of oxidative stress and heat shock proteins. ACS Books, Oxidative Stress: Diagnostics and Therapy. Editors: E. Silvana Andreescu & Maria Hepel;1083 (12), 341-367.
 
Woods, A.G., Sokolowska, I., Yakubu, R., Butkiewicz, M., LaFleur, M., Talbot, C., Darie, C.C. (2011). Blue Native PAGE and mass spectrometry as an approach for the investigation of stable and transient protein-protein interactions. ACS Books, Oxidative Stress: Diagnostics and Therapy. Editors: E. Silvana Andreescu & Maria Hepel;1083 (13), 369-411.
 
Darie C.C., Deinhardt, K.I., Zhang G., Cardasis H.L., Chao., Neubert T.A. (2011). Identifying transient protein-protein interactions in EphB2 signaling by Blue Native PAGE and mass spectrometry. Proteomics; 11(23): 4514-28.
 

Darie C.C., Litscher E. S., Wassarman P. M. (2008). Structure, processing, and polymerization of rainbow trout egg vitelline envelope proteins. Applications of Mass Spectrometry in Life Safety. NATO Science for Peace and Security Series. Springer-Verlag, Düsseldorf, Germany, pp 23-36


Darie C.C., Shetty V., Spellman D.S., Zhang G., Xu C., Cardasis H.L., Blais S., Fenyo D., Neubert T.A. (2008). Blue Native PAGE and mass spectrometry analysis of the ephrin stimulation- dependent protein-protein interactions in NG108-EphB2 cells. Applications of Mass Spectrometry in Life Safety. NATO Science for Peace and Security Series. Springer-Verlag, Düsseldorf, Germany, pp 3-22


Spellman D.S., Deinhardt K, Darie, C.C., Chao, M.V., Neubert, T.A. (2008). Stable Isotopic Labeling of Amino Acids in cultured primary neurons: application to BDNF-dependent phosphotyrosine-associated signaling.signal. Mol. Cell. Proteomics. 7 (6):1067-1076


Darie C.C., Janssen W.G., Litscher E.S., Wassarman P.M. (2008). Purified trout egg vitelline envelope proteins VEβ and VEγ polymerize into homomeric fibrils from dimers in vitro. Biochim. Biophys. Acta - Proteins and Proteomics; 1784 (2):385-392


Litscher E.S., Janssen W.G., Darie C.C., Wassarman P.M. (2008). Purified mouse egg zona pellucida glycoproteins polymerize into homomeric fibrils under non-denaturing conditions. J. Cel. Physiol.; 214 (1): 153-157


Darie C.C., De Pascalis L., Mutschler B., Haehnel W. (2006). Studies of the Ndh complex and photosystem II from mesophyll and bundle sheath chloroplasts of the C4-type plant Zea mays. J. Plant Physiol.; 163 (8): 800-808


Darie C.C., Biniossek M.L., Gawinowicz M.A., Milgrom Y, Thumfart J.O., Jovine L., Litscher E.S., Wassarman P.M. (2005). Mass spectrometric evidence that proteolytic processing of rainbow trout egg vitelline envelope proteins takes place on the egg. J. Biol. Chem. 280 (45): 37585-37598


Darie C.C., Biniossek M.L., Winter, V, Mutschler B., Haehnel W. (2005). Isolation and structural characterization of the Ndh complex from mesophyll and bundle sheath chloroplasts of Zea mays. FEBS Journal 272: 2705-2712


Jovine L., Darie C.C., Litscher E.S., Wassarman P.M. (2005). ZP domain proteins and glycoproteins. Annu. Rev. Biochem. 74: 83-114


Wassarman P.M., Jovine L., Qi H., Williams Z., Darie C., Litscher E. (2005). Recent aspects of mammalian fertilization research. Mol. Cell. Endocrinol. 234 (1-2): 95-103


Darie C.C., Biniossek M.L., Jovine L., Litscher E.S., Wassarman P.M. (2004). Structural characterization of fish egg vitelline envelope proteins by mass spectrometry. Biochemistry  43: 7459-7478


Darie C., Drepper F., Mutschler B., Mauch S., Hoerth P., Biniossek M., Haehnel W. (2001). Studies of cyclic electron transport in bundle sheath chloroplasts and isolation of the NDH complex from chloroplasts of Zea mays. Proceedings of the 13th Congress of Photosynthesis, S14-002


Artenie V., Godja A., Naum E., Darie C. (1999). Glutathione-peroxidase activity from sanguine serum in patients with different mental diseases. J. Med. Biochem. (J. Cell. Mol. Med.) 3: 73-78


Darie C., Jerca L., Artenie V., Margina A. (1998). Glutathione peroxidase, an oxidative stress biomarker, in the menstrual cycle. Rev. Med. Chir. Soc. Med. Nat. 102: 138-144
Current Biochemistry & Proteomics Group members:
  • Izabela Sokolowska, MS, Ph.D. student
  • Armand Gatien Ngounou Wetie, MS., Ph.D. student
  • Alisa G. Woods, Ph.D., Research Assistant Professor

Current Undergraduate Students

  • Jessica Wagner, Clarkson 2012
  • Jeannette Dorler, Clarkson 2012
  • Kelly Wormwood, Clarkson 2014

Alumni:

  • Rama Yakubu, Clarkson 2011 
  • Supriya Mathur, MS., Ph.D. student 
  • Melissa Butkiewicz, Clarkson 2011 
  • Aditya Thiagarajan, Clarkson 2012 
  • Martin LaFleur, Clarkson 2013 
  • Stefanie Russell, SUNY Plattsburgh 2012 
  • Andrew Wegner, Clarkson 2015 
  • Jonathan Samson, Clarkson 2010 
  • Laura Zielewicz, Clarkson 2014 
  • Dan Rivera, Clarkson 2010 
  • Ann Marie Selva, Clarkson 2011
  •  Christopher Talbot, Clarkson 2010