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Costel C. Darie

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darie

Costel C. Darie
Assistant Professor
Chemistry & Biomolecular Science
147 Science Center

Clarkson University
PO Box 5810
Potsdam, NY 13699-5810
Phone: 315-268-7763
E-mail: cdarie@clarkson.edu

Website:
http://people.clarkson.edu/~cdarie/index.html


Educational Background

2002 Ph.D., Biochemistry, University of Freiburg, Germany
1999 MS, Biochemistry, Al.I. Cuza University, Iasi, Romania
1997 BS, Biochemistry, Al.I. Cuza University, Iasi, Romania
1991 RN, Vaslui County Hospital, Vaslui, Romania


Professional Experience

2008-2009    Associate Scientist, Mount Sinai School of Medicine, New York, NY, USA
2006-2008    Research Scientist, Skirball Institute, New York University, New York, NY, USA
2003-2006    Postdoctoral Associate, Mount Sinai School of Medicine, New York, NY, USA


Research Interests
Our research interests focus on three main areas: 1) understanding the molecular mechanisms of protein-protein interactions and how they potentially relate to disease states and 2) investigating the molecular mechanisms of cell proliferation and differentiation 3) identifying serum biomarkers for diagnostic and prognostic of cancer. In our studies, we mainly use a combination of analytical biochemistry, protein chemistry and mass spectrometry.

 
Current Biochemistry & Proteomics Group members:
  • Armand Gatien Ngounou Wetie, MS., Ph.D. student
  • Roshanak Aslebagh, MS, Ph.D. student
  • Sapan Patel, MS, Ph.D. student
  • Kelly Wormwood, Clarkson 2013, Ph.D. student
  • Devika Channaveerappa, M.S., Ph.D. Student 
  • Alisa G. Woods, Ph.D., Research Assistant Professor
  • Urmi Roy, Ph.D., Adjunct Research Associate Professor
 

Current Undergraduate Students:

  • Mary Williams 
  • Ashley Brisbin
  • Megan Borland 
  • Emmalyn J. Dupree 
 

Alumni:

  • Izabela Sokolowska, Ph.D. 2013
  • Daniela Hutanu, Ph.D. 
  • Loredana Lupu, BS 2014, MS student 
  • Adrian Robu, BS 2014, MS student 
  • Kelly Wormwood, Clarkson 2013
  • Katherine M. Beglinger, Clarkson 2014
  • Coleman Larlee, US Army ROTC, Clarkson 2013
  • Jarrod W. Mattingly, Clarkson 2014
  • Bethany M. Wawro, Clarkson 2014
  • Ledwin Amaro, Clarkson 2014
  • Rachel A. Skavina, Clarkson 2014
  • Earl Cash, Clarkson 2015
  • Jessica Wagner, Clarkson 2012
  • Jeannette Dorler, Clarkson 2012
  • Rama Yakubu, Clarkson 2011
  • Melissa Butkiewicz, Clarkson 2011 
  • Aditya Thiagarajan, Clarkson 2012 
  • Martin LaFleur, Clarkson 2013 
  • Andrew Wegner, Clarkson 2015 
  • Jonathan Samson, Clarkson 2010 
  • Laura Zielewicz, Clarkson 2014 
  • Dan Rivera, Clarkson 2010 
  • Ann Marie Selva, Clarkson 2011
  • Christopher Talbot, Clarkson 2010
  • Stefanie Russell, SUNY Plattsburgh 2012 
  • Supriya Mathur, MS., Ph.D. student 
  • Blake Woods, High School Jr. Glen Cove, NY

Book:

http://www.springer.com/chemistry/analytical+chemistry/book/978-3-319-06067-5

book

Selected Publications (published at Clarkson):

71. Robu, A., Lupu, L., Aslebagh, R., Zamfir, A.D., Darie, C.C. (2014). Advances in mass spectrometry for glycoscreening and sequencing in biomedical research. Mod. Chem. Appl., 2: 138. doi:10.4172/2329-6798.1000138.

70. Woods, A.G., Wormwood, K.L., Ngounou Wetie, A.G., Aslebagh, R., Crimmins, B.S., Holsen, T.M., Darie, C.C. (2014). Autism Spectrum Disorders: an omics perspective. Proteomics Clin Appl. 2014 Oct 14. doi: 10.1002/prca.201400116. [Epub ahead of print].

69. Hutanu D, Frishberg MD, Guo L, Darie CC (2014). Recent Applications of Polyethylene Glycols (PEGs) and PEG Derivatives. Mod Chem Appl., 2: 132. doi:10.4172/2329-6798.1000132.

68. Wormwood, K.L., Dupree, E.J.*, Darie, C.C., Woods, A.G. (2014). Environmental ,nfluences on Biochemistry in Autism Spectrum Disorder. Autism-Open Access 4: e123. doi:10.4172/2165-7890.1000e123.

67. Hutanu, D., Darie, C.C. (2014). Trends in Characterization of PEGylated Proteins by Mass Spectrometry. Mod Chem Appl., 2: 2: 128. doi:10.4172/2329-6798.1000128.

66. Roy, U., Woods, A.G., Sokolowska, I., and Darie, C.C. (2014). Structural investigation of HSP70-HSP90 and HSP90-TDF interactions. Mod. Chem. Appl., 2: 126, doi: 10.4172/2329-6798.1000126

65. Ngounou Wetie, A.G., Wormwood, K.L.*, Thome, J., Dudley, E., Tarines, R., Gerlach, M., Woods, A.G., Darie, C.C. (2014). Potentially Interactive Oxidative Stress and Cholesterol Dysregulation Protein Markers are Elevated in Autism Spectrum Disorder, Electrophoresis. 35(14):2046-54. doi: 10.1002/elps.201300370.

64. Andrei, V., Ngounou Wetie, A.G., Mihai, I., Darie, C.C., Vasilescu, A. (2014). Detection of biomedically relevant stilbenes from wines by mass spectrometry. Advancements of Mass Spectrometry in Biomedical Research, Eds. Alisa G. Woods & Costel C. Darie, Advances in Experimental Medicine and Biology Series (ISSN: 0065-2598), Springer International Publishing Switzerland, 806: 361-82.

63. Woods, A.G., Iosifescu, D.V., Darie, C.C. (2014). Biomarkers in Major Depressive Disorder: the role of mass spectrometry. Advancements of Mass Spectrometry in Biomedical Research, Eds. Alisa G. Woods & Costel C. Darie, Advances in Experimental Medicine and Biology Series (ISSN: 0065-2598), Springer International Publishing Switzerland, 806: 545-60.

62. Ngounou Wetie, A.G., Woods, A.G., Darie, C.C. (2014). Mass spectrometric analysis of Post-translational modifications (PTMs) and protein-protein interactions (PPIs). Advancements of Mass Spectrometry in Biomedical Research, Eds. Alisa G. Woods & Costel C. Darie, Advances in Experimental Medicine and Biology Series (ISSN: 0065-2598), Springer International Publishing Switzerland, 806: 205-35.

61. Schneider, S.S., Aslebagh, R., Ngounou Wetie, A.G., Sturgeon, S.R., Darie, C.C., Arcaro, K.F. (2014). Using Breast Milk to Assess Breast Cancer Risk: The Role of Mass Spectrometry-Based Proteomics. Advancements of Mass Spectrometry in Biomedical Research, Eds. Alisa G. Woods & Costel C. Darie, Advances in Experimental Medicine and Biology Series (ISSN: 0065-2598), Springer International Publishing Switzerland, 806: 399-408.

60. Sandu, C., Ngounou Wetie, A.G., Darie, C.C., Steller, H. (2014). Thiostrepton, a natural compound that triggers heat shock response and apoptosis in human cancer cells: a proteomics investigation. Advancements of Mass Spectrometry in Biomedical Research, Eds. Alisa G. Woods & Costel C. Darie, Advances in Experimental Medicine and Biology Series (ISSN: 0065-2598), Springer International Publishing Switzerland, 806: 443-51.

59. Baral, R., Ngounou Wetie, A.G., Darie, C.C., Wallace, K. (2014). Mass Spectrometry for Proteomics-based Investigation using the Zebrafish vertebrate model system. Advancements of Mass Spectrometry in Biomedical Research, Eds. Alisa G. Woods & Costel C. Darie, Advances in Experimental Medicine and Biology Series (ISSN: 0065-2598), Springer International Publishing Switzerland, 806: 331-40.

58. Branza-Nichita, N., Petrareanu, C., Lazar, C., Sokolowska, I., Darie, C.C. (2014). Using proteomics to unravel the mysterious steps of the HBV life-cycle. Advancements of Mass Spectrometry in Biomedical Research, Eds. Alisa G. Woods & Costel C. Darie, Advances in Experimental Medicine and Biology Series (ISSN: 0065-2598), Springer International Publishing Switzerland, 806: 453-81.

57. Patel, S., Ngounou Wetie, A.G., Darie, C.C., Clarkson, B.D. Cancer Secretomes and their Place in Supplementing other Hallmarks of Cancer (2014). Advancements of Mass Spectrometry in Biomedical Research, Eds. Alisa G. Woods & Costel C. Darie, Advances in Experimental Medicine and Biology Series (ISSN: 0065-2598), Springer International Publishing Switzerland, 806: 409-42.

56. Woods, A.G., Sokolowska, I., Deinhardt, K., Darie, C.C. (2014). Investigating a novel protein using mass spectrometry: the example of Tumor Differentiation Factor (TDF). Advancements of Mass Spectrometry in Biomedical Research, Eds. Alisa G. Woods & Costel C. Darie, Advances in Experimental Medicine and Biology Series (ISSN: 0065-2598), Springer International Publishing Switzerland, 806: 509-23.

55. Ngounou Wetie, A.G., DeKroon, R.M., Mocanu, M., Ryan, J.P., Darie, C.C., Woods, A.G. (2014). Mass Spectrometry for the Study of Autism and Neurodevelopmental Disorders. Advancements of Mass Spectrometry in Biomedical Research, Eds. Alisa G. Woods & Costel C. Darie, Advances in Experimental Medicine and Biology Series (ISSN: 0065-2598), Springer International Publishing Switzerland, 806: 525-44.

54. Roy, U., Woods, A.G., Sokolowska, I., Darie, C.C. (2014). Utility of Computational Structural Biology in Mass Spectrometry. Advancements of Mass Spectrometry in Biomedical Research, Eds. Alisa G. Woods & Costel C. Darie, Advances in Experimental Medicine and Biology Series (ISSN: 0065-2598), Springer International Publishing Switzerland, 806: 107-28.

53. Ngounou Wetie, A.G., Shipp, D.A., Darie, C.C. (2014). Bottlenecks in proteomics. Advancements of Mass Spectrometry in Biomedical Research, Eds. Alisa G. Woods & Costel C. Darie, Advances in Experimental Medicine and Biology Series (ISSN: 0065-2598), Springer International Publishing Switzerland, 806: 581-93.

52. Woods, A.G., Sokolowska, I., Ngounou Wetie, A.G., Wormwood, K., Aslebagh, R., Patel, S. Darie, C.C. (2014). Mass Spectrometry for Proteomics-based Investigation. Advancements of Mass Spectrometry in Biomedical Research, Eds. Alisa G. Woods & Costel C. Darie, Advances in Experimental Medicine and Biology Series (ISSN: 0065-2598), Springer International Publishing Switzerland, 806: 1-32.

51. Sokolowska, I., Ngounou Wetie, A.G., Wormwood, K.*, Thome, J., Darie, C.C., Woods, A.G. Biomarkers in psychiatry (2013). The potential of biomarkers in psychiatry: focus on proteomics. J. Neural Transm. 1-10. doi: 10.1007/s00702-013-1134-6.

50. Wormwood, K.L.*, Sokolowska, I., Ryan, J.P., Russell, S.*, Darie, C. C., Woods, A. G. (2013). The Potential for Proteomics in Understanding Neurodevelopmental Disorders. J. Proteomics Bioinform. S5. http://dx.doi.org/10.4172/jpb.S5-001.

49. Roy, U., Sokolowska, I., Woods, A.G., and Darie, C.C. (2013) Tumor Differentiation Factor (TDF) and its Receptor (TDF-R): Is TDF-R an Inducible Complex with Multiple Docking Sites? Mod Chem appl 1:108. doi: 10.4172/2329-6798.1000108.

48. Roy, U., Woods, A.G., Sokolowska, I., Darie, C.C. (2013). Structural Evaluation and Analyses of Tumor Differentiation Factor. Protein J., 32(7):512-8. doi: 10.1007/s10930-013-9510-5.

47. Beglinger, K.M.*, Wormwood, K.L.*, Mattingly, J.W.*, Larlee, C.R.*, Woods, B.**, Wawro, B.*, Darie, C.C. (2013) Mass Spectrometry in an Undergraduate Setting: Clarkson University. Mod Chem appl 1:112. doi: 10.4172/2329-6798.1000112.

46. Hutanu, D., Woods, A.G., Darie, C.C. (2013). Recent Applications of Mass Spectrometry in Paint Analysis. Mod Chem appl 1:109. doi:10.4172/2329-6798.1000109.

45. Beglinger, K.M.*, Zielewicz, L.*, Ngounou Wetie, A.G., Darie, C.C. (2013). Analysis of proteins bound to contact lenses. Mod Chem appl 1:4.http://dx.doi.org/10.4172/2329-6798.1000114

44. Wormwood, K.L.*, Ngounou Wetie, A.G., Beglinger, K.M.*, Mattingly, J.W.*, Larlee, C.R.*, Woods, A.G., Darie, C. C. (2013). Using mass spectrometry to investigate protein biomarkers. Analele Stiintifice ale Universitatii Al. I. Cuza Iasi, Sectiunea IIA, Genetica si Biologie Moleculara, 14:3.

43. Darie C.C. (2013). Post-Translational Modification (PTM) Proteomics: Challenges and Perspectives. Mod Chem appl 1:e114. doi:10.4172/2329-6798.1000e114.

42. Woods, A. G., Wormwood, K.L.*, Ngounou Wetie, A.G., Ryan, J.P., Darie, C. C. (2013). Proteomics and cholesterol in autism 3:2.
http://dx.doi.org/10.4172/2165-7890.1000112

41. Petrareanu, C., Macovei, A., Sokolowska, I., Woods, A.G., Darie, C.C., Lucian, D., Branza-Nichita N (2013). Comparative Proteomics Reveals Novel Components at the Plasma Membrane of Differentiated HepaRG Cells and Different Distribution in Hepatocyte- and Biliary-Like Cells. PLoS ONE 8(8): e71859. doi:10.1371/journal.pone.0071859

40. Darie C.C. (2013). Investigation of Protein-Protein Interactions by Blue Native-PAGE & mass spectrometry. Mod Chem appl 1: e111. doi:10.4172/mca.1000e111.

39. Ngounou Wetie, A.G., Sokolowska, I., Wormwood, K.L.*, Beglinger, K.*, Michel, T.M., Thome, J., Darie, C.C., Woods, A.G. (2013). Mass spectrometry methods for the detection of psychiatric biomarkers. Journal of Molecular Psychiatry, 1:8; doi:10.1186/2049-9256-1-8

38. Woods, A. G., Ngounou Wetie, A.G., Sokolowska, I., Russell, S.*, Ryan, J.P., Michel, T.M., Thome, J., Darie, C. C. (2013). Mass spectrometry as a tool for studying autism spectrum disorder. Journal of Molecular Psychiatry, 1: 6; doi:10.1186/2049-9256-1-6.

37. Darie, C.C. (2013). Mass Spectrometry and its Applications in Life Sciences. Australian Journal of Chemistry, 66, 719-720; DOI:10.1071/CH13284.

36. Ngounou Wetie, A.G., Sokolowska, I., J., Woods, A.G., Darie, C.C. (2013). Identification of post-translational modifications by mass spectrometry. Australian Journal of Chemistry, 66, 734-748; DOI: 10.1071/CH13144.

35. Sokolowska, I., Ngounou Wetie, A.G., Woods, A.G., Darie, C.C. (2013) Applications of mass spectrometry in proteomics. Australian Journal of Chemistry, 66, 721-733; DOI: 10.1071/CH13137.

34. Darie C.C. (2013). Mass Spectrometry and Proteomics: Principle, Workflow, Challenges and Perspectives. Mod Chem appl 1: e105. doi:10.4172/mca.1000e105.

33. Woods, A.G., Sokolowska, I., Deinhardt, K., Sandu, C., Darie, C.C. (2013). Identification of Tumor Differentiation Factor (TDF) in select CNS neurons. Brain structure and function, 70:2835-2848; doi: 10.1007/s00429-013-0571-1.

32. Ngounou Wetie, A.G., Sokolowska, I., Woods, A.G., Roy, U., Deinhardt, K., Darie, C.C. (2013) Protein-protein interactions: Switch from classical methods to proteomics-based approaches. Cellular and Molecular Life Sciences. doi: 10.1007/s00018-013-1333-1.

31. Florian, P.F., Macovei. A., Lazar, L., Milac, A.L., Sokolowska, I., Darie, C.C., Evans, R.W., Roseanu, A., Branza-Nichita, N. (2013). Characterization of the anti-HBV activity of lactoferrin-derived peptides. Journal of Medical Virologydoi: 10.1002/jmv.23549.

30. Sokolowska, I., Ngounou Wetie, A.G., Roy, U., Woods, A.G., Darie, C.C. (2013) Mass spectrometry investigation of glycosylation on the NXS/T sites in recombinant glycoproteins. Biochimica et Biophysica Acta. doi: 10.1016/j.bbapap.2013.04.022.

29. Ngounou Wetie, A.G., Sokolowska, I., Woods, A.G., Roy, U., Loo, J.A., Darie, C.C. Investigation of stable and transient protein-protein interactions: past, present and future. Proteomicsdoi: 10.1002/pmic.201200328

28. Roy, U., Sokolowska, I., Woods, A.G., Darie, C.C. (2012). Structural investigation of tumor differentiation factor. Biotechnology and Applied Biochemistrydoi: 10.1002/bab.1046

27. Sokolowska, I., Woods, A.G., Gawinowicz, M.A., Roy, U., Darie, C.C. (2012). Characterization of tumor differentiation factor (TDF) and its receptor (TDF-R). Cellular and Molecular Life Sciences. 70 (16), 2835-2848 doi: 10.1007/s00018-012-1185-0

26. Ngounou, A. G., Sokolowska, I., Woods, A. G., Wormwood, K. L., Dao, S., Patel, S., Clarkson, B. D., Darie, C.C. (2012) Automated Mass Spectrometry-based functional assay for the routine analysis of the secretome. Journal of Laboratory Automation. 18(1):19-29. DOI: 10.1177/2211068212454738.

25. Sokolowska, I., Woods, A. G., Gawinowicz, M. A., Roy, U., Darie, C.C. (2012) Identification of a potential tumor differentiation factor (TDF) receptor from steroid-responsive and steroid-resistant prostate cancer cells. FEBS Journal, 279 (14): 2579-94, DOI: 10.1111/j.1742-658.2012.08641.

24. Sokolowska, I., Gawinowicz, M. A., Ngounou, A. G., Darie, C.C. (2012). Disulfide proteomics for identification of extracellular or secreted proteins. Electrophoresis. 33(16):2527-36. DOI: 10.1002/elps.201200182.

23. Sokolowska, I., Ngounou, A. G., Woods, A. G., Darie, C.C. (2012). Automatic determination of disulfide bridges in proteins. Journal of Laboratory Automation. 17(6):408-16. DOI: 10.1177/2211068212454737.

22. Sokolowska, I., Dorobantu, C., Woods, A.G., Macovei, A., Branza-Nichita N, Darie, C.C. (2012). Proteomic analysis of plasma membranes isolated from undifferentiated and differentiated HepaRG cells. Proteome Science, 10:47, DOI: 10.1186/1477-5956-10-47.

21. Woods, A.G., Sokolowska, I., Darie, C.C. (2012). Identification of consistent alkylation of cysteine-less peptides in a proteomics experiment.Biochemical and Biophysical Research Communications, 419 (2): 305-8.

20. Sokolowska, I., Woods, A.G., Gawinowicz, M.A., Roy, U., Darie, C.C. (2012). Identification of a potential tumor differentiation factor (TDF) receptor from steroid-responsive and steroid-resistant breast cancer cells. J. Biol. Chem., 287(3): 1719-33.

19. Woods A.G., Sokolowska I., Taurines R., Gerlach M., Dudley E., Thome J., Darie C.C. (2012) Potential biomarkers in psychiatry: focus on the cholesterol system. Journal of Cellular and Molecular Medicine. 16(6):1184-95. doi: 10.1111/j.1582-4934.2012.01543.

18. Thome J., Coogan A.N., Woods A.G., Darie C.C., Häßler F. (2011). CLOCK Genes and Circadian Rhythmicity in Alzheimer Disease. J Aging Res., 2011: 383091 doi:10.4061/2011/383091.

17. Sokolowska, I., Woods, A.G., Wagner, J.*, Dorler, J.*, Wormwood, K.*, Thome, J., Darie, C.C. (2011). Mass spectrometry for proteomics-based investigation of oxidative stress and heat shock proteins. ACS Books, Oxidative Stress: Diagnostics and Therapy. Editors: E. Silvana Andreescu & Maria Hepel; 1083 (12), 341- 367

16. Woods, A.G., Sokolowska, I., Yakubu, R.*, Butkiewicz, M.*, LaFleur, M.*, Talbot, C.*, Darie, C.C. (2011). Blue Native PAGE and mass spectrometry as an approach for the investigation of stable and transient protein-protein interactions. ACS Books, Oxidative Stress: Diagnostics and Therapy. Editors: E. Silvana Andreescu & Maria Hepel; 1083 (13), 369-411.

15. Darie C.C., Deinhardt, K.I., Zhang G., Cardasis H.L., Chao., Neubert T.A. (2011). Identifying transient protein-protein interactions in EphB2 signaling by Blue Native PAGE and mass spectrometry. Proteomics; 11(23): 4514-28.

14. Darie C.C., Litscher E. S., Wassarman P. M. (2008). Structure, processing, and polymerization of rainbow trout egg vitelline envelope proteins. Applications of Mass Spectrometry in Life Safety. NATO Science for Peace and Security Series. Springer-Verlag, Düsseldorf, Germany, pp 23-36

13. Darie C.C., Shetty V., Spellman D.S., Zhang G., Xu C., Cardasis H.L., Blais S., Fenyo D., Neubert T.A. (2008). Blue Native PAGE and mass spectrometry analysis of the ephrin stimulation- dependent protein-protein interactions in NG108-EphB2 cells. Applications of Mass Spectrometry in Life Safety. NATO Science for Peace and Security Series. Springer-Verlag, Düsseldorf, Germany, pp 3-22

12. Spellman D.S., Deinhardt K, Darie, C.C., Chao, M.V., Neubert, T.A. (2008). Stable Isotopic Labeling of Amino Acids in cultured primary neurons: application to BDNF-dependent phosphotyrosine-associated signaling.signal. Mol. Cell. Proteomics. 7 (6):1067-1076

11. Darie C.C., Janssen W.G., Litscher E.S., Wassarman P.M. (2008). Purified trout egg vitelline envelope proteins VEðb and VEðg polymerize into homomeric fibrils from dimers in vitro. Biochim. Biophys. Acta - Proteins and Proteomics; 1784 (2):385-392